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Superoxide creation are detected within the bloodstream and you can BALF by using the CMH twist probe. Blood otherwise BALF products were obtained away from PBS- and you will Bleo-addressed mice and incubated instantaneously having CMH. The fresh samples were relocated to the newest PTFE pipe and you will flash suspended, and you will EPR analysis are accumulated from the 77 K. The brand new intensity of nitroxide (CM.) gathered in the bloodstream incubated having CMH (0.2 mM) during the 37 levels to own ten min (Profile 4A).

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